MKV file not played using samba

You could try Settings -> Services -> SMB Client, there is a new setting: “Fix issues MTU issues with SMBv2" while I am not sure if that is the problem.
If it still doesn’t work after that and you want to dig deeper you would need to enable debug logging and smb component specific logging and provide those logs.

Alternatively you might want to switch to kernel based mounting as described here: